Calculadora de Diluição Serial

The Serial Dilution Calculator determines the final concentration of a solution after multiple serial dilutions. The process involves transferring a fixed volume aliquot from an initial solution into a container with a larger volume of diluent, mixing, and repeating the procedure with the new diluted solution. The formula used is Cₙ = C₀ × (Va/Vt)ⁿ, where C₀ is the initial concentration, Va is the aliquot volume transferred, Vt is the total volume of the new solution (Va plus diluent volume), and n is the number of dilutions. This tool is essential in chemistry, biology, and pharmacy laboratories for preparing solutions with precise and decreasing concentrations.

Practical uses include preparing standards for calibration curves in spectrophotometry, microbiological sensitivity tests, and toxicology experiments. For example, a researcher can dilute a 1 mg/mL sample through a series of 10 dilutions, each with a 1:10 dilution factor, obtaining concentrations of 0.1 mg/mL, 0.01 mg/mL, and so on. The calculator streamlines this calculation, preventing manual errors and ensuring reproducibility. It is important to note that the dilution factor (Va/Vt) must be constant at each step for the series to be uniform.

Common precautions include thorough homogenization after each dilution and using calibrated volumetric glassware for accuracy. The calculator assumes ideal dilutions without losses or contamination. For series with different factors, step-by-step calculation is required. Additionally, the final concentration should never be below the detection limit of the analytical method used. This tool is free and online, available for any user needing quick and reliable serial dilution calculations.