UFC/m³ Ambiente Estéril

≤10.
Created by
Renato Passos, Eng. de Software
Reviewed by
Renato Passos, Eng. de Software

Last updated: Apr 18, 2026

UFC/m³
10

About this calculator

The Environment Contamination Calculator is an essential tool for professionals working in sterile environments, such as laboratories, operating rooms, and sterile product production areas. It calculates environmental contamination in Colony-Forming Units per cubic meter (CFU/m³), a critical parameter for ensuring sterility and safety in these environments.

The formula used is based on the count of CFU in an air sample collected in a specific volume. The result is expressed in CFU/m³, allowing professionals to assess whether the environment meets the required sterility standards. Values below 10 CFU/m³ are considered acceptable for sterile environments.

It is crucial to use this calculator in cases where contamination can have serious consequences, such as in the production of sterile drugs, vaccines, and other products. Additionally, it is useful in investigations of hospital infection outbreaks, helping to identify sources of contamination.

When using the calculator, it is important to be careful with the calibration of sampling equipment and the correct execution of the CFU counting technique. Furthermore, it is essential to consider environmental variables, such as temperature and humidity, which can influence the results.

Frequently asked questions

What does CFU/m³ mean?

CFU/m³ means Colony-Forming Units per cubic meter. It is a measure of microbial contamination in the air.

Why is the limit ≤10 CFU/m³?

The limit of ≤10 CFU/m³ is considered acceptable for sterile environments because it minimizes the risk of contamination and infection.

How is the air sample collected?

The air sample is collected using specific equipment, such as air sampling pumps and filters or Petri dishes.

What are the precautions when using the calculator?

It is important to calibrate the sampling equipment and correctly execute the CFU counting technique. Additionally, consider environmental variables.

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