Título de anticorpo

Título = maior diluição positiva.
Created by
Renato Passos, Eng. de Software
Reviewed by
Renato Passos, Eng. de Software

Last updated: Apr 18, 2026

título
512

About this calculator

The antibody titer calculator is a tool used to determine the highest dilution of a serum or solution that still shows a positive reaction in an antibody detection test. The titer is an important measure in immunology as it helps assess the concentration of antibodies present in a sample. This is done through serial dilutions of the sample, testing each dilution to see if there is still a reaction with the corresponding antigen.

The calculator works based on the dilution formula, expressed as a ratio between the initial concentration of the sample and the applied dilution. For example, if a sample has a titer of 1:100, it means the sample was diluted 100 times and still showed a positive reaction. The calculator helps determine this value quickly and accurately.

This calculator is particularly useful in cases of diagnosing infectious diseases, monitoring immune response in patients treated with vaccines or hyperimmune sera, and in scientific research involving antibody detection. Care must be taken with sample preparation and test execution, as these factors can directly influence the antibody titer result.

Attention to common precautions is essential, such as precision in preparing dilutions, the use of calibrated equipment for testing, and correct interpretation of results. Additionally, it is crucial to consider the specificity and sensitivity of the test used to detect antibodies.

Frequently asked questions

What does a high antibody titer mean?

A high antibody titer means that the sample contains a large amount of antibodies against a specific antigen. This may indicate an active or recent infection, or an effective immune response.

How is dilution performed for titer calculation?

Dilution is performed serially, starting with a low dilution and gradually increasing until the positive reaction is no longer observed. The titer is the highest dilution that still shows a positive reaction.

What are the precautions when performing the antibody titer test?

It is important to use calibrated equipment, follow precise dilution protocols, and perform quality controls to ensure the accuracy of the results.

When is antibody titer used in clinical practice?

Antibody titer is used to diagnose and monitor infectious diseases, assess immune response to vaccines or hyperimmune sera, and in scientific research.

What can affect the antibody titer result?

Factors such as sample quality, precision in dilution preparation, and test execution can influence the antibody titer result.

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